[Objectives] Exocrine glands distributed throughout the skin are one of the conspicuous characteristics in amphibians. The secretions of these glands play a vital role in reproduction, communication, and defense. In order to investigate the types, distribution, and ultrastructure of the glands in treefrogs, we used histological techniques to observe the microstructure (including Hematoxylin-Eosin, Alcian Blue-Periodic Acid Schiff and Masson staining methods) and ultrastructure of skin samples from cephalic, dorsal, lateral, mental and ventral regions of Hainan Frilled Treefrogs (Kurixalus hainanus). [Methods] A linear mixed effect model was used to analyze differences in thickness of skin, distribution of glands and size of glands among body regions in sex-specific individuals where body regions were used as a fixed factor and individuals were used as a random factor, and sex differences of those metrics in specific body regions where sex was used as a fixed factor and individuals were used as a random factor. All statistics were performed in SPSS 22.0. [Results] Ordinary mucous glands (Ⅰ MG & Ⅱ MG), serous glands, but not specialized glands were found in most areas (Fig. 1). Microstructure analysis revealed that both male and female frogs had the same type of glands, but there were significant differences in the skin thickness, the distribution of glands, and the size of glands among body regions in sex-specific individuals (Fig. 2, 3). In addition, there was a significant sex difference in these metrics in specific body regions (Fig. 2, 3). The differences in skin thickness might result from an adaption to the locomotive and reproductive patterns of K. hainanus. The differences in distribution and size of the glands might be related to defensive function and sexual dimorphism. Ultrastructural analysis showed that there were different sizes and densities of vesicles in mucous glands (Fig. 4), and the aggregate serous vesicles indicated a staged reaction of substance maturation (Fig. 5). [Conclusion] This study provides fundamental information on skin structure, types of glands, and distribution of glands in anurans. Moreover, this study contributes to further study on the chemical component of volatile secretions from K. hainanus with a morphological basis.