Lycodon pictus was described from Cao Bang Province, Northern Vietnam by Janssen et al. 2019. In 2020, it was confirmed to have a distribution in Nonggang National Nature Reserve, Longzhou County, Guangxi Zhuang Autonomous Region, China, which is the first recorded from China. In September 2021, two snake specimens (one male and one female) of the genus Lycodon were collected in Libo County, Qiannan Buyi and Miao Autonomous Prefecture, Guizhou Province, China (25°14′3″ N, 107°55′32″ E, altitude 530 m) during a field survey. Based on the morphological comparison and phylogenetic analyses, the collected specimens of the genus Lycodon were identified as L. pictus,which is a new record to Guizhou Province. The specimens were fixed in 10% formalin for one day, subsequently kept permanently in 75% ethanol, and deposited in Anhui Normal University Museum (ANUM). The fresh liver tissue was extracted and immediately preserved in 95% ethanol. 31 sequences of 18 recognized species of genus Lycodon and2 outgroups (Boiga cynodon, Dipsadoboa flavida) were downloaded from GenBank (Accession numbers listed in Fig. 2). Uncorrected pairwise distances (p-distance) among closely related congeners were calculated in MEGA X software. Phylogenetic tree was constructed by maximum likelihood (ML) in RaxML 7.2.6 software. After external morphological (Fig. 1) and morphometric measurements (Table 1) examination, the specimens from Libo County were similar to Lycodon pictus. Molecular phylogenetic analyses revealed that the uncorrected pairwise distances (p-distance) between the two collected specimens and L. pictus were from 0.3% to 0.8% (Table 2). The new distribution site of L. pictus in Libo, Guizhou, China is approximately 350 km away from the type locality in Vietnam. The new discovery in the present study expands the distribution area of L. pictus, and it also enriches the species diversity of the genus Lycodon in Guizhou Province.