[Objectives] Urban green spaces are vital components of the urban landscape and hold significant ecological and cultural values. Studying the impact of human activities on bird diversity can provide a theoretical basis for constructing and maintaining ecological urban landscapes. [Methods] This study used the transect method to investigate bird communities in 8 urban green spaces with 3 disturbance levels in Wuhan, China from October 2021 to May 2022. The bird community structure was analyzed using diversity parameters, including species richness, diversity index, evenness index, and dominance index. Differences were assessed using one-way ANOVA. [Results] A total of 100 bird species in 11 orders and 34 families were recorded, including 9 nationally protected birds species at the II-level (Appendix 1). Passerines comprised the majority (76 species, 76% of the total bird species surveyed) of the recorded species, with the most important fauna and resident type were oriental birds (45 species, 45%) and resident birds (42 species, 42%), respectively. During the breeding season, the number of bird species is higher than that during the non-breeding season, primarily due to an increase in summer visitors and passage migrant. Among the bird communities in green spaces with varying disturbance intensities, the highest species richness, Shannon-Wiener index, and uniformity index were observed in mildly disturbed areas, while the species richness, Shannon-Weiner index, and uniformity index are the lowest in areas with moderate interference (Fig. 1). The dominance index was highest in highly disturbed areas, and lowest in mildly disturbed areas (Fig. 1). During different seasons, there are differences in bird diversity and community structure, but seasons do not affect the pattern of bird communities across different levels of human disturbance. [Conclusion] In summary, the bird diversity of urban green spaces in Wuhan is relatively rich, and there was a nonlinear relationship with disturbance intensity, indicating that the difference between human disturbance and natural disturbance in small and fragmented patches may be more conducive to maintaining biodiversity.