[Objectives] Little Gland Frog Glandirana minima is an endemic amphibian species in Fujian, China. It is evaluated as Critically Endangered (CR) in the Red List of Vertebrates in China, as well as Endangered (EN) by IUCN. It is listed as the second-class state key protected wildlife in China. Little Grand Frog has an extremely small population, but currently, it is not covered by any nature reserve in Fujian Province, facing a great risk of extinction. Moreover, since its first report in the late 1970s, there has been very little research available, which is not conducive to the implementation of relevant protection measures. Conducting field investigations and reassessing the endangered level of this species help to gain a deeper understanding of its survival and expansion capabilities in the wild, and to provide scientific data for formulating conservation strategies for the G. minima. [Methods] From July 2019 to August 2021, a continuous survey was conducted on the distribution, quantity, reproductive behavior, habitat, and microenvironment of the G. minima in its historical distribution points and the surrounding area of Fuzhou using visual encounter surveys and call counting methods. The number of the G. minima at each distribution point was determined by selecting the maximum count obtained from multiple surveys, accounting for variations in survey frequencies at each point. The total number of G. minima was determined by aggregating the counts of at each distribution point. The key identification features of the G. minima were determined through the utilization of image capture techniques and direct field observations. At the same time, according to the evaluation criteria of the IUCNRed List of Threatened Levels and Standards (version 3.1), a reassessment of the endangered level of the G. minima was conducted based on survey data. [Results] The main recognition feature of the G. minima is the longitudinal skin ribs with varying lengths in 8 rows on the back and the side (Fig. 1a and c), consistent with the description of the type specimen. Juveniles are similar to adults, with only shorter longitudinal skin edges (Fig. 1f). The population distribution shows significant changes compared to the historical records. Among the four historical distribution points of the G. minima (black triangles in Fig. 2), only the type specimen location (Lingshi Temple, Fuqing) still has the distribution of the G. minima (the southernmost black triangles in Fig. 2), and three new village level distribution points have been discovered around it; we did not find the distribution of the G. minima in three other historical sites (Yongquan Temple in Fuzhou, Fangguangyan in Yongtai, and Wenshu Temple in Changle), but a total of eight new village level distribution points have been discovered in other parts of their administrative regions (Fig. 2). In addition, 11 village level distribution points in 5 counties were newly found outside the administrative regions of historical distribution points (Fig. 2). Overall, there are 22 new village level distribution points added compared to historical distribution points. The area of the distribution region is 4 800 km² (Fig. 2), which is six times the size of the historical distribution region. The suitable habitats for the G. minima are waterlogged fields, streams, ponds, small pool and mountainous areas with an altitude of 10﹣740 m (Table 1), with abandoned rice paddies being the main habitat (Table 1). The population size shows a characteristic that depends on the suitable habitat area, with a total of 459 ind among all distribution points (Table 1). Among them, 82.6% are distributed in areas with elevations above 100 m (Table 1). The preferred habitats are broad-leaved forests (Table 1). The breeding period is from May to July, and tadpoles can undergo metamorphosis in the same year or the following year. The reevaluation results show that the threatened state of the G. minima is between Endangered (EN) and Vulnerable (VU). [Conclusion] The G. minima has strong adaptability to its habitat. However, anthropogenic changes in the water environment are the main threat faced by the G. minima. Drought can reduce the number of G. minima. Therefore, protecting the abandoned rice paddies, their microhabitats, and water sources, on which they rely for survival are key focuses of future conservation work. The G. minima is only distributed in a narrow region in Fuzhou and its northern area, further research is needed to reveal the internal mechanism of its narrow distribution.