湖南沅江鼎城段河蚬的性别组成与精子形态特征
作者:
作者单位:

1.湖南农业大学水产学院 长沙 410128;2.湖南应用技术学院农林科技学院 常德 415100

作者简介:

王旭,男,硕士;研究方向:渔业资源与环境;E-mail:2381340243@stu.hunau.edu.cn。

基金项目:

国家自然科学基金项目(No. 31772832),湖南省现代农业产业技术体系项目(湘农发〔2019〕105号),湖南省科学技术厅重点研发项目(No. 2022NK2060);


Sex Composition and Sperm Morphological Characteristics of Corbicula fluminea from the Section Dingcheng of Yuan River in Hunan Province, China
Author:
Affiliation:

1.Fisheries College, Hunan Agricultural University, Changsha 410128; 2.College of Agriculture and Forestry, Hunan Applied Technology University, Changde 415100, China

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [43]
  • |
  • 相似文献 [7]
  • | | |
  • 文章评论
    摘要:

    本研究以湖南沅江鼎城段河蚬(Corbicula fluminea)为研究对象,在进一步验证该群体性别组成的基础上,分析壳长组成与性别比例之间关系,探讨该群体雄性、雌性和雌雄同体之间关系;同时以单鞭毛精子为参照,分析比较双鞭毛精子的形态特征,以期为我国河蚬的性别发生及生殖适应策略研究提供基础资料。结果显示:沅江鼎城段河蚬(n = 770)雄性、雌性和雌雄同体最小性成熟壳长分别为2.92 mm、5.66 mm和5.30 mm。697只性成熟河蚬中雄性、雌性和雌雄同体的比例近似1︰1︰6。雌雄同体的平均壳长[(22.55 ± 0.33)mm,n = 517]显著大于雄性[(20.44 ± 1.03)mm,n = 95]和雌性[(19.79 ± 0.99)mm,n = 85](P < 0.05),但雄性与雌性的平均壳长之间差异不显著(P > 0.05)。河蚬可以产生单鞭毛和双鞭毛两种类型的精子,单鞭毛精子头长范围4.93 ~ 21.79 μm,平均值(14.27 ± 0.82)μm(n = 30),双鞭毛精子头长范围10.29 ~ 22.04 μm,平均值(15.62 ± 0.62)μm(n = 26)。单、双鞭毛精子头长差异不显著(P > 0.05)。双鞭毛精子(n = 26)长尾的平均长度[(38.07 ± 1.44)μm]显著大于其短尾[(31.08 ± 1.60)μm]和单鞭毛精子(n = 30)尾部长度[(30.15 ± 1.75)μm](P < 0.01),但其短尾与单鞭毛精子的尾部平均长度之间无显著性差异(P > 0.05)。结果表明:湖南沅江鼎城段河蚬为雄性先熟,且可能存在雄性和(或)雌性向雌雄同体转换现象。河蚬具有单鞭毛型和双鞭毛型2种类型的精子,且双鞭毛精子的2个尾部不等长。同域共存河蚬的单鞭毛与双鞭毛精子在运动及受精能力方面的差异值得深入研究。

    Abstract:

    [Objectives] To provide basis for future research on gender generation and reproductive adaptation strategies of Corbicula clams, the sex composition of C. fluminea in the section Dingcheng of Yuan River, Hunan Province, was verified, and the potential sexual transition among male, female and hermaphrodites was also explored based on their shell length. In addition, the morphological characteristics was compared between monoflagellate and biflagellate sperms. [Methods] Histological techniques were used to analyze the sex composition and the morphology of sperms. The differences of head length between monoflagellate and biflagellate sperms were compared using T-test. One-way ANOVA was used to analyze the shell length among sexes and the length differences among tails of sperms, and multiple comparisons were performed using least-significant difference (LSD). [Results] The minimum shell length at sexual maturity is 2.92 mm in males, 5.66 mm in females, and 5.30 mm in hermaphrodite individuals, respectively (n = 770), and their sex ratio was approximately 1︰1︰6 (n = 697) (Fig. 3). The average shell length of hermaphrodites (22.55 ± 0.33 mm) was significantly greater than that of the males (20.44 ± 1.03 mm) (P < 0.05) and the females (19.79 ± 0.99 mm) (P < 0.01), but there was no significant difference between males and females (P > 0.05) (Table 1). The C. fluminea population in the Yuan River can produce both monoflagellate and biflagellate sperms. The head length of monoflagellate sperms ranged from 4.93 to 21.79 μm, with a mean value of 14.27 ± 0.82 μm, and that of biflagellate sperms ranged from 10.29 to 22.04 μm, with a mean value of 15.62 ± 0.62 μm, with no significant difference between these two types of sperms (P > 0.05) (Table 2). The average length of long tails (38.07 ± 1.44 μm) was significantly greater than that of short tails (31.08 ± 1.60 μm) and the tails of monoflagellate sperms (30.15 ± 1.75 μm) (P < 0.01), while no significant difference in average flagella length was observed between the short tails and the tails of monoflagellate sperms (P > 0.05) (Table 2). [Conclusion] Our results indicated that the C. fluminea population in the section Dingcheng of Yuan River was protandrous, and with possible transition from male and/or female to hermaphrodite. There are two types of sperms:monoflagellate and biflagellate sperm with different tail length. The differences in motility and fertilization ability between coexisting monoflagellate and biflagellate sperm are worthy of further study.

    参考文献
    Araujo R, Moreno D, Ramos M. 1993. The Asiatic clam Corbicula fluminea (Müller, 1774) (Bivalvia:Corbiculidae) in Europe. American Malacological Bulletin, 10(1):39–49.
    Bespalaya Y V, Bolotov I N, Aksenova O V, et al. 2018. Aliens are moving to the Arctic frontiers:an integrative approach reveals selective expansion of androgenic hybrid Corbicula lineages towards the North of Russia. Biological Invasions, 20(8):2227–2243.
    Boryshpolets S, Kholodnyy V, Cosson J, et al. 2018. Fish sperm motility analysis:the central role of the flagellum. Reproduction, Fertility, and Development, 30(6):833–841.
    Byrne M, Phelps H, Church T, et al. 2000. Reproduction and development of the freshwater clam Corbicula australis in southeast Australia. Hydrobiologia, 418(1):185–197.
    Cao L, Damborenea C, Penchaszadeh P E, et al. 2017. Gonadal cycle of Corbicula fluminea (Bivalvia:Corbiculidae) in Pampean streams (Southern Neotropical Region). PLoS ONE, 12(10):e0186850.
    Crespo D, Dolbeth M, Leston S, et al. 2015. Distribution of Corbicula fluminea (Müller, 1774) in the invaded range:a geographic approach with notes on species traits variability. Biological Invasions, 17(7):2087–2101.
    Eckelbarger K J, Bieler R, Mikkelsen P M. 1990. Ultrastructure of sperm development and mature sperm morphology in three species of commensal bivalves (Mollusca:Galeommatoidea). Journal of Morphology, 205(1):63–75.
    Elliott P, Zu Ermgassen P S E. 2008. The Asian clam (Corbicula fluminea) in the River Thames, London, England. Aquatic Invasions, 3(1):54–60.
    Franzén ?. 1983. Ultrastructural studies of spermatozoa in three bivalve species with notes on evolution of elongated sperm nucleus in primitive spermatozoa. Gamete Research, 7(3):199–214.
    Graf D L. 2013. Patterns of freshwater bivalve global diversity and the state of phylogenetic studies on the Unionoida, Sphaeriidae, and cyrenidae. American Malacological Bulletin, 31(1):135–153.
    Howard D R, Trantow C M, Thaler C D. 2004. Motility of a biflagellate sperm:waveform analysis and cyclic nucleotide activation. Cell Motility and the Cytoskeleton, 59(2):120–130.
    Ishibashi R, Komaru A. 2003. Invasion of Corbicula fluminea into the Lake Biwa-Yodo River System. Venus, 62:65–70.
    Jun J, Kim B, Chung E, et al. 2009. Spermatogenesis and ultrastructural characteristics of spermatozoa of brackish water diploid clam, Corbicula japonica (Bivalvia:Corbiculidae). The Korean Society of Developmental Biology, 13(2):115–122.
    Komaru A, Konishi K. 1996. Ultrastructure of biflagellate spermatozoa in the freshwater clam, Corbicula leana (Prime). Invertebrate Reproduction & Development, 29(3):193–197.
    Konishi K, Furuita K K H, Komaru A. 1998. Spermatogenesis of the freshwater clam Corbicula aff. fluminea Müller (Bivalvia:Corbiculidae). Journal of Shellfish Research, 17(1):185–189.
    Korniushin A V. 2004. A revision of some Asian and African freshwater clams assigned to Corbicula fluminalis (Müller, 1774) (Mollusca:Bivalvia:Corbiculidae), with a review of anatomical characters and reproductive features based on museum collections. Hydrobiologia, 529(1):251–270.
    Lee T, Siripattrawan S, Ituarte C F, et al. 2005. Invasion of the clonal clams:Corbicula lineages in the new world. American Malacological Bulletin, 20(1/2):113–122.
    Morton B. 1977. The population dynamics of Corbicula fluminea (Bivalvia:Corbiculacea) in Plover Cove Reservoir, Hong Kong. Journal of Zoology, 181(1):21–42.
    Morton B. 1983. The sexuality of Corbicula fluminea (Müller) in lentic and lotic waters in Hong Kong. Journal of Molluscan Studies, 49(1):81–83.
    Moueza M, Frenkiel L. 1995. Ultrastructural study of the spermatozoon in a tropical lucinid bivalve:Codakia orbicularis L. Invertebrate Reproduction & Development, 27(3):205–212.
    Niijima L, Dan J. 1965. The acrosome reaction in Mytilus edulis. I. Fine Structure of the intact acrosome. The Journal of Cell Biology, 25(2):243–248.
    Pi J, Tang Y X, Coughlan N E, et al. 2023. Temperature drives reproductive activity in a rare trioecy population of Corbicula clams. Hydrobiologia, 1–15.
    Pigneur L M, Marescaux J, Roland K, et al. 2011. Phylogeny and androgenesis in the invasive Corbicula clams (Bivalvia, Corbiculidae) in western Europe. BMC Evolutionary Biology, 11:147.
    Qiu A, Shi A, Komaru A. 2001. Yellow and brown shell color morphs of Corbicula fluminea (Bivalvia:Corbiculidae) from Sichuan Province, China, are triploids and tetraploids. Journal of Shellfish Research, 20(1):323–328.
    Robb-Chavez S B, Bollens S M, Rollwagen-Bollens G, et al. 2022. Broadscale distribution, abundance, and habitat associations of the invasive Asian clam (Corbicula fluminea) in the lower Columbia River, USA. International Review of Hydrobiology, 107(5/6):179–195.
    Sousa M, Corral L, Azevedo C. 1989. Ultrastructural and cytochemical study of spermatogenesis in Scrobicularia plana (Mollusca, Bivalvia). Gamete Research, 24(4):393–401.
    Sousa R, Antunes C, Guilhermino L. 2008. Ecology of the invasive Asian clam Corbicula fluminea (Müller, 1774) in aquatic ecosystems:an overview. International Journal of Limnology, 44(2):85–94.
    Suquet M, Labbe C, Brizard R, et al. 2010. Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm. Theriogenology, 74(1):111–117.
    Vastrade M, Etoundi E, Bournonville T, et al. 2022. Substantial genetic mixing among sexual and androgenetic lineages within the clam genus Corbicula. Peer Community Journal, 2:e73.
    Zeng C, Tang Y X, Vastrade M, et al. 2023. Salinity appears to be the main factor shaping spatial COI diversity of Corbicula lineages within the Chinese Yangtze River Basin. Diversity and Distributions, 29(3):364–378.
    郭延平, 谈奇坤, 陈士超. 2002. 三角帆蚌精子的形态及超微结构. 动物学杂志, 37(2):10–13.
    赖俊任, 蔡奇立, 郭金全. 2020. 在台湾的亚洲蚬(Corbicula fluminea)其生殖腺构造和性比. 台湾生物多样性研究, 22(1):1–12.
    刘燕, 李英. 2004. 黄蚬(Corbicula aurea heude)及蚬属其它三种的比较生物学研究. 贵阳金筑大学学报, (1):93–96.
    刘月英, 张文珍, 王跃先. 1979. 中国经济动物志:淡水软体动物. 北京:科学出版社, 120.
    裴宏谦, 占江凡, 秦玲, 等. 2020. 洞庭湖流域沅江蚬属贝类的倍性研究. 生命科学研究, 24(6):452–458, 465.
    王改改, 王家雄, 沈丽燕, 等. 2018. 精子鞭毛形态与精子运动参数的关系. 中国医药导报, 15(27):12–16, 181.
    王剑平, 李德亮, 曾聪, 等. 2018. 捞刀河浏阳段河蚬的遗传多样性及生殖特征研究. 水生生物学报, 42(5):913–918.
    王庆志, 常亚青. 2010. 大洋河河蚬的繁殖生物学研究. 大连水产学院学报, 25(1):8–13.
    占江凡. 2020. 长江中下游湖泊蚬的遗传结构与性别特征. 湖南农业大学硕士学位论文.
    占江凡, 裴宏谦, 李科静, 等. 2021. 沅江常德段蚬属贝类的谱系、倍性与性别特征. 水生生物学报, 45(1):125–131.
    赵雪琳, 郑小东, 孙同秋, 等. 2012. 黄河三角洲河蚬(Corbicula fluminea)的繁殖生物学研究. 海洋与湖沼, 43(5):1008–1015.
    郑玉林, 王银东, 卢文轩. 1997. 河蚬的性别和性腺发育的研究. 安徽农业科学, 25(2):84–85.
    周小龙, 董迎辉, 边平江, 等. 2012. 帘文蛤精子超微结构及与其他双壳贝类的比较. 台湾海峡, 31(4):495–500.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

王旭,皮杰,刘林崴,唐杨欣,刘新华,向建国,李德亮.2024.湖南沅江鼎城段河蚬的性别组成与精子形态特征.动物学杂志,59(3):387-396.

复制
文章指标
  • 点击次数:964
  • 下载次数: 9400
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2023-07-12
  • 在线发布日期: 2024-06-17