中国林蛙早期胚胎发育及其与近缘种的比较
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河南师范大学生命科学学院,河南省太行山森林生态系统野外科学观测研究站 新乡 453007

作者简介:

张澍卉,女,硕士研究生;研究方向:动物资源与系统进化;E-mail:zshui2455@163.com。

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国家自然科学基金联合基金重点支持项目(No. U21A20192),中央财政国家重点野生动植物保护资助项目;


Early Embryonic Development of Rana chensinensis and Comparison with Its Related Species
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College of Life Sciences, Henan Normal University, Field Scientific Observation and Research Station of Forest Ecosystem in Taihang Mountains, Henan Province, Xinxiang 453007, China

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    摘要:

    为探究中国林蛙(Rana chensinensis)早期胚胎发育特征,2022年3月收集其在河南省济源市太行山区野外自然抱对产出的受精卵,在室内水温(22.63 ± 2.09)℃的条件下进行连续观察。结果显示,中国林蛙卵径(1.57 ± 0.01)mm(n = 5);早期胚胎发育分为25个时期,从受精卵到鳃盖完成期历时158.83 h;卵裂方式为不均等全裂;大约70%的胚胎在鳃血循环期出膜。中国林蛙早期胚胎发育的卵裂特征、出膜方式及出膜时期与近缘种相似,速度较慢;与其他无尾两栖类相比,卵裂特征、出膜方式和发育速度均呈现种属特异性。中国林蛙较小的卵径、较快的早期胚胎发育速度反映了其对静水产卵环境的适应。

    Abstract:

    [Objectives] The embryonic development of amphibians is an important research field of developmental biology and ecology. Rana chensinensis is an important economic amphibian in China, and its early embryonic development needs to be supplemented. This study explored the characteristics of early embryonic development of R. chensinensis. [Methods] The fertilized eggs produced by natural clutches in the Taihang Mountains of Jiyuan City, Henan Province were continuously observed under the condition of water temperature 22.63 ± 2.09 ℃ indoor with stereomicroscope. The embryonic development was observed, and the duration of each stage, the starting time of each stage of development, water temperature, observation times, embryonic morphology and physiological characteristics were recorded and photographed. [Results] The results showed that the egg diameter of R. chensinensis was 1.57 ± 0.01 mm (n = 5), and the early embryonic development was divided into 25 stages. It took 158.83 h from fertilized egg to gill operculum completion period. The cleavage mode was unequal complete cleavage, and about 70% of the embryos outed of the membrane in the gill blood circulation stage. The completion process of early embryonic development was recorded and described (Table 1, Fig. 1). [Conclusion] The cleavage characteristics, hatching mode and hatching period of early embryonic development of R. chensinensis are similar to those of related species, but the development speed is slower. Compared with other anuran amphibians, its cleavage characteristics, the way of out membrane and development speed reflect species specificity. The smaller egg diameter and faster early embryonic development speed reflect the adaptation to shallow and still water spawning habitats in R. chensinensis (Table 2, Fig. 2).

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张澍卉,高书轩,侯银梦,杨欣玥,朱艳军,陈晓虹.2024.中国林蛙早期胚胎发育及其与近缘种的比较.动物学杂志,59(4):570-578.

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  • 收稿日期:2023-09-18
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  • 在线发布日期: 2024-08-23
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