[Objectives] Guangdong Province, located in southern China and the subtropical and tropical zone of East Asia, has a highly diverse fauna of amphibians and reptiles, which attracted a number of research efforts consistently. However, some previous studies were imperfect in quality, and an overall and detailed report of the herpetofauna in Guangdong is still absent. Therefore, researchers from School of Life Sciences / School of Ecology, Sun Yat-sen University (Code:SYS), conducted a series of field investigations in numerous localities in Guangdong recently, and obtained many amphibian and reptile specimens. Morphological examinations and molecular phylogenetic analyses on these specimens were employed, to determine if there were newly recorded species in Guangdong among them. [Methods] From 2018 to 2023, a total of 810 amphibian and 391 reptile specimens from Guangdong were collected and deposited in SYS. Morphological characters of these specimens were examined and measured in digital vernier caliper (Deli DL91200 Stainless Hardened, 0 to 200 mm, being accurate to 0.1 mm) to primarily identify the species, then Bayesian inference trees were constructed using MEGA 11, jModeltest, and MrBayes 3.2.4, and uncorrected p-distance were calculated using MEGA 11, based on mitochondrial 16S rRNA, COI, ND2,andCyt b genes, to confirm the species delimitation. [Results] We confirmed eight species as new records of amphibians and reptiles in Guangdong (Fig. 1), including Pachytritonwuguanfui (collected in Giant Salamander Provincial Natural Reserve, Liannan, Guangdong), Bombina fortinuptialis (collected in Mt. Dawuling, Xinyi, Guangdong), Boulenophrys xiangnanensis (collected in Giant Salamander Provincial Natural Reserve, Liannan, Guangdong), Odorrana wuchuanensis (collected in Jiangying Town Forest Station, Yangshan, Guangdong), Nidirana xiangica (collected in Mt. Dadongshan, Lianzhou, Guangdong), Theloderma corticale (collected in Mt. Nanling, Ruyuan, Guangdong), Gekko palmatus (collected in Mt. Dinghushan, Zhaoqing, Guangdong), and Pareas stanleyi (collected in Mt. Nanling, Ruyuan, Guangdong). Morphological characters of these newly collected specimens in Guangdong were described and measured (Table 1﹣4), and their phylogenetic relationships were also analyzed (Appendix 1﹣16). [Conclusion] The amphibian and reptile fauna of Guangdong was preliminarily made clear, but due to the imbalanced and inadequate herpetological research in Guangdong previously, especially in western, northwestern, and northeastern regions, the diversity of amphibians and reptiles in this province is still underestimated. In summary, the herpetofauna of Guangdong needs further investigations and conservations in the future.