[Objectives] The characteristics of resource utilization, niche differentiation and coexistence mechanism of sympatric species were considered the research focuses in the field of ecology, and those studies have the guiding significance for the conservation of endangered species and biodiversity protection. [Methods] From July to August 2018, the line-transect methods and quadrat sampling method were adopted to investigate the habitat characteristicof sympatric speciesincluding Tetraogallus himalayensis, Pseudois nayaur, Marmota bobak, and Capra hircus in Dongdashan Nature Reserve, Gansu Province. A total of 6 ecological factors were measured to explore the differences in habitat use among the four species. We also adopted the niche breadth and overlapping index of habitats’ ecological factors to measure the degree of niche overlap among four species. Some statistical testing methods were used for data analysis:one-sample Kolmogorov-Smirnov Z test for normality test of data; one-way analysis of variance (ANOVA) was used when data satisfied normal assumption for comparison among multiple independent samples, and Kruskal-Wallis test was used when data not satisfied normal assumption; independent sample T-test was used when data were satisfied normal assumption for comparing with two independent samples, and instead, Mann-Whitney U test was used when data were not satisfied normal assumption; principal component analysis (PCA) and kernel density estimation were used for presenting niche differentiation degree among the four species. [Results] We found 108, 56, 110 and 42 locations respectively for T. himalayensis, P. nayaur, M. bobak and C. hircus in Dongdashan Nature Reserve (Fig. 1). The results of statistical analysis showed that the ecological factors were significantly different among the four species (Table 1), and the scatter plots of the principal component score of habitat ecological factors for different species indicated there were obvious niche differentiations, but there were still niche overlaps (Fig. 2). The niche breadth and overlapping index of 6 ecological factors among different species showed that T. himalayensis held two eurytopic factors that the value of trophic niche breadth bigger than 0.8, while P. nayaur held five eurytopic factors, M. bobak held three factors and C. hircus with two. The overlapping index of altitude, slope, aspect, and distance to the road between T. himalayensis and C. hircusare were higher than the indexes between other animals, which showed that the effect of grazing. The number of eurytopic ecological factors of P. nayaur and M. bobak was greater than T. himalayensis, and frequently appeared in the territory of T. himalayensis, which showed that similarity of habitat selection (Table 2, 3). In particular, the niche breadth and overlapping indexes of ecological factors among four species can be directly reflected by the kernel density functions, and we also found significant overlap between T. himalayensis and other animals in some specific intervals of ecological variables (Fig. 3). [Conclusion] With regard to the particular resources preference and narrow niche selected, as well as the last surviving core distribution area of T. himalayensis in Dongdashan Nature Reserve, although other three animals had more or less different habitat preferences, due to their wider niche and probabilistic co-occurrence in same locations, the potential mutually competition among different species was probably remains.