Abstract:During the amphibian survey in Wulong District, Chongqing City, five specimens were collected on 2 August, 2022 (29°32′29″ N, 107°53′15″ E, altitude 697 m) (Fig. 1). Compared the specimens with other species of the genus Odorrana by morphological and molecularevidences(Table 1), the specimens were identified as Odorrana kweichowensis, which is the new record of Chongqing, China. Morphologically, the specimens collected from Wulong appeared to be similar with O. kweichowensis (Fig. 2), the snout vent length of the males and females collected from Wulong were longer than that of the topotype (Table 2); molecularly, we constructed phylogenetic trees using Bayesian inference (BI) and maximum likelihood (ML) methods based on ND2 gene and calculated the genetic distances between species of the genus Odorrana using Kimura 2-parameter model in MEGA v6.0. The BI and ML trees based on ND2 gene presented almost consistent topology, showing that the five specimens from Chongqing were clustered with O. kweichowensis with high support (1.00/100) (Fig. 3). The genetic distance between the specimens from Chongqing and O. kweichowensis from type locality was 0.03%, much lower than that between Odorrana species, ranging from 5.56% to 24.38% (Table 3). The new record of O. kweichowensis in Chongqing would promote taxonomic and phylogeographic studies on this species.