Abstract:The Achalinus dabieshanensis was initially described as a new species in 2023. It is only known to be located in Anhui Province, China. This study provides morphological and molecular information of A. dabieshanensis, which was collected in Huangbaishan National Forest Park, Henan Province, China. In this study, 22 morphological indexes were selected for morphological comparison. The length was measured by vernier caliper, and the number of scales was described manually. Genomic DNA was extracted from liver tissue (preserved in 95% alcohol) using FastPure Gel DNA Extraction Mini Kit, cytochrome c oxidase subunit I (COI) was targeted and amplified using primers Chmf4 and Chmr4. Sequences of 21 recognized species of genus Achalinus and one outgroup (Fimbrio klossi) were downloaded from GenBank (Table 1). Uncorrected pairwise distances (p-distance) among closely related congeners were calculated using MEGA 7.0 software. Phylogenetic tree was constructed by Bayesian inference (BI) analysis using PhyloSuite v1.2.2 software. The body measurements and scale morphology of the collected specimen showed little difference compared with A. dabieshanensis from type locality. The temporal of this specimen is 1/(1 + 1) + 2 + 2, which is morphologically different from the temporal (2 + 2 + 3) of the topotype (Fig. 1). Phylogenetic tree indicated that the specimen closely clustered with a topotype of A. dabieshanensis (Lu’an, Anhui, China) (Fig. 2). The genetic distance between them is 0.002, significantly smaller than the interspecific genetic distance of Achalinus (0.034﹣0.297) (Appendix 1). The ecological environment of the specimen was similar to that of the topotype (Fig. 3). Considering both the morphological characteristics and the molecular phylogenetic relationship, the specimen of Achalinus snake, HNNU2307I021, from Xinyang County, Henan Province was identified as A. dabieshanensis,which was a new provincial record of Henan.