One specimen of Lycodon was collected from Chawalong Town, Zayu County, Tibet Autonomous Region, China during filed work in August and September 2018. The specimen from Zayu was similar to holotype for morphological comparison (Table 2). Analysis of mitochondrial DNA Cyt b sequences revealed that the sample from Zayu cluster with samples from Lushui and Lincang, Yunnan Province. The genetic distance within this clade was limited in 0.004﹣0.018 (Table 3). The specimen from Zayu was more related with specimens from Lushui with a minor distance between 0.004﹣0.006. Combined with morphological comparison, the Zayu specimen was finally identified as L. gongshan. This was the first record for the species in Tibet Autonomous Region. We also presented description of the hemipenes morphology of L. gongshan for the first time. Hemipenes of L. gongshan near bulbous, covered with small spine; spine on hemipenis larger and keratinized on base; no calyculate zone on top. Hemipenes not bifurcate; sulcus shallow, distinct, without spines; reaches 5th subcaudals when everted (Fig. 3). Two known body coloration of the species were recorded: rufous with black banded from Lushui and the type locality Gongshan County, Yunnan Province; white with black banded from Panzhihua in Sichuan Province. However, the body of Zayu specimen are olive-brown with black banded, which is reported for the first time.