In order to explore the effects of hypoxia-acidification stress on the growth and physiological metabolism of Larimichthys crocea in the early development stages, 4 treatment groups were designed, namely the control group [dissolved oxygen (DO) = 7.0 mg/L, pH 8.1], the hypoxia group (DO = 3.5 mg/L, pH 8.1), acidification group (DO = 7.0 mg/L, pH 7.3), and hypoxia-acidification group (DO=3.5mg/L, pH 7.3). Four replicates were conducted in each treatment group, and 4.0 × 104 fertilized eggs were placed in each repetition.The contents of insulin like growth factor 1 (IGF-Ⅰ) and growth hormone (GH), and the activities of pyruvate kinase (PK), glutamic pyruvic transaminase (GPT), alkaline phosphatase (AKP) and Na+-K+-ATPase (NKA) were determined after the treatment (recorded as the 0th d) and the 1st d, 3rd d, 5th d, 10th d, 15th d, 20th d and 27th d after hatching. Lengths and heights of bodies in each group on 27th d were also determined. Both hypoxia and acidification stress significantly inhibited the growth of body length and height on the 27th d, and the inhibitory effect of hypoxia-acidification stress was more significant. The contents of IGF-I were significantly lower in the three treatment groups at many time points than in the control group (P < 0.05), and the contents of IGF-I in the three treatment groups were significantly lower than in the control group on the 3rd d (P < 0.05). The contents of GH showed an increasing trend in different treatment groups, and were higher than in the control group on the 27th d (P < 0.05). The activity of PK of the hypoxia-acidification group was significantly higher than that in the control group (P < 0.05) from the 1st to the 5th d. The changes in the other three groups basically showed a trend of increasing first and then decreasing. The activities of GPT in the 3 treatment groups were significantly higher than in the control group at many time points (P < 0.05). The activities of AKP of the 3 treatment groups were significantly lower than in the control group on the 3rd d (P < 0.05), and were significantly higher than in the control group on the 15th d (P < 0.05). The activities of NKA in the 3 treatment groups were significantly lower thanin the control group on the 3rd d (P < 0.05). Conclusion: Under the experimental conditions, the individual growth of L. crocea in the early stage in the environment of hypoxia and acidification stressis inhibited, and the dual stress of hypoxia and acidification has a more significant effect. Compared with the control group, the activities of metabolic enzymes of each treatment group changesignificantlyat many time points. Combining the analysis of the individual growth differences and metabolic enzyme activities of L. crocea in this experiment, it is concluded that hypoxia and acidification stress cause responsive changes in the activities of the main metabolism enzymes , which ultimately affects the individual growth of L. crocea.