Based on the field investigations in 30 counties of Yunnan Province between 2001 and 2015, the present paper analyzed the infestation and related ecological issues of mites (gamasid mites and chigger mites) on the body surface of Mole Shrews (Anourosorex squamipes) in the province. The constituent ratio (Cr), prevalence (PM), mean abundance (MA) and mean intensity (MI) of mites on A. squamipes shrews were calculated in a conventional way. Jaccard’s similarity coefficient (J) was calculated to compare the similarity of mite species. Margalef’s index (Mf) was used to measure the species richness of mites. The DF, DG and DG-F were used to measure the species diversity of mites at family and genus levels. The patchiness index (m*/m) and K parameter of negative binomial distribution (k) were used to determine the spatial distribution pattern of dominant mite species among different individuals of A. squamipes shrews. The association coefficient (V) was used to measure the mutual relationship between chigger mites (trombiculid mites) and gamasid mites. A total of 348 mole shrews (A. squamipes) were captured from 10 of 30 investigated counties in Yunnan. The collected 15 541 mites from A. squamipes were identified as 103 species belonging to 24 genera and 10 families. The Mf species richness of chigger mites was higher than that of gamasid mites, but all species diversity indices of gamasid mites at family and genus levels (DG = 2.80, DF = 2.32 and DG-F = 0.17) were higher than those of chigger mites (DG = 1.43, DF = 1.26 and DG-F = 0.12). The infestation of A. squamipes shrews with mites was common with a high overall prevalence (PM = 80.75%), mean abundance (MA = 44.66 mites/shrew) and mean intensity (MI = 55.31 mites/shrew). The PM, MA and MI of gamasid mites were obviously higher than those of chigger mites (PM: χ2 = 182.580, P < 0.001; MA: Z =﹣12.495, P< 0.001; MI: Z =﹣0.371, P > 0.05). A positive association existed between chigger mites and gamasid mites (V = 0.18, P < 0.001). Dipolaelaps anourosorecis (Cr = 92.79%) was the dominant species of gamasid mites and Leptotrombidium densipunctatum (Cr = 23.10%) was the dominant species of chigger mites. The two dominant mite species were of aggregated distribution among different individuals of their animal host, A. squamipes (m*/m > 1, k > 0). Different sexes and different ages of A. squamipes had different mite infestations, and more mites tended to choose male and adult A. squamipes shrews. The species similarity of chigger mites was relatively low on male and female A. squamipes shrews (J = 0.31), but that of gamasid mites was relatively high (J = 0.65). The species similarity of chigger mites was very low on different ages of A. squamipes shrews (J = 0.17), and that of gamasid mites was also low (J = 0.28). The infestation of A. squamipes shrews with mites is common with heavy infestation intensity and very high species diversity of mites. On A. squamipes shrews, L. densipunctatum is the dominant species of chigger mites and D. anourosorecis is the dominant species of gamasid mites, and they are of aggregated distribution. There are sex-bias and age-bias in the infestations of A. squamipes shrews with mites.