[Objectives] Amphibians and reptiles are good environmental indicator. Recently, multiple endemic new species were discovered in Eastern Tibet, China, however their habitat is under threat. It is important to have adequate knowledges about the regional herpetological biodiversity and spatial distribution for biodiversity protection. [Methods] In order to obtain these information, we conducted a series of field surveys in Basu, Zuogong, and Mangkang Counties of eastern Tibet from August 2019 to July 2020. Within the altitude range of 2 270﹣4 610 m, a total of 155 transects with a length of 100﹣500 m were investigated (Fig. 1). There are 42 transects in Basu, 49 in Zuogong and 64 in Mangkang. Species, individual number, vegetation types and elevation of each transects were recorded. Shannon - Weiner index of different vegetation types, encounter rate and dominance for species were analyzed. [Results] There are eight amphibians species belonging to five genera and four families, seven reptile species belonging to four genera and three families were recorded in the surveyed region area (Table 1). Detailed geographical distribution maps for amphibians and reptiles were drawn and shown in Fig. 2 & 3 respectively. The results of Shannon - Weiner index among the five vegetation types indicates that the order of biodiversity is bush > coniferous forest > broad-leaved forest > mixed coniferous broad-leaved forest > meadow (Table 2). The elevational distribution range of amphibians is 2 280﹣4 350 m, while that of reptiles is 2 270﹣3 470 m (Fig. 4). The fauna of amphibians and reptiles in the survey area of eastern Tibet are quite different. Most amphibians are distributed in both Palaearctic and Oriental realms, but the majority of reptiles are Oriental. There are three species Diploderma vela, D. batangense, and D. laeviventre listed Garde II in the National Key Protected Wild Animal List. In addition, we report a new record of reptilian fauna of Tibet Autonomous Region, Protobothrops xiangchengensis. [Conclusion] The longitudinal valleys and rivers in the surveyed region in eastern Tibet are possible explanations for the differences of amphibian and reptile fauna. It is suggested that the dry-hot valley should be considered in the planning and construction of the nature reserve, where all the three species listed in Garde II in the List of National Key Protected Wild Animals are distributed.