Species distribution is important for studying biodiversity, as it provides the essential recognition for biogeography characteristics. The number of recognized distribution areas of the genus Barbastella in the family Vespertilionidae of Chiroptera has increased over recent years. According to the morphological and ecological indices, different species of the genus are usually distinguished. On August 16th, 2019, one male of Barbastella was captured by using mist net in Lei Sheng Village, Tongmin Town, Xishui County, Guizhou Province, China. Its forearm length is 36.16 mm. Its ears are squarish with distinctive transverse ridges. The ears are forward facing and join across the forehead. There is no lobe protruding from the outer edge of each ear. Tragus length (6.92 mm) is nearly a half of ear length (13.81 mm); the tragus is triangular (Fig. 1, Table 1). As a result, it is identified as Asian barbastelle B. leucomelas which representing a new record species in Guizhou Province, China.