[Objectives] In order to reveal the differences in the main morphological characteristics of the Leptobrachella, it is hoped to provide a reference for the subsequent taxonomy and pedigree geography of Leptobrachella. [Methods] Apply morphological analysis methods, use the shortest distance method of Euclidean distance for cluster analysis, construct a cluster tree to show the grouping relationship of six species. The stepwise discriminant method is used for discriminant analysis, the trait parameters that contribute greatly to the discrimination are selected, and the discriminant formulas of six species of Leptobrachella are established. The morphological characteristic values of the individuals to be determined are respectively substituted into the discriminant formulas, and the formula values are calculated and compared. The largest is the population to which the individual of Leptobrachella belongs, so as to verify the ability of each main deformation contribution trait to distinguish species. [Results] The results indicated that: Morphological character Snout-vent length comparison: From large to small, the snout-vent length of the six species of Leptobrachella are L. chishuiensis, L. suiyangensis, L. jinshaensis, L. bijie, L. wulingensis and L. purpuraventra (Table 3); Cluster analysis showed that among the 6 species, the difference between the L. purpuraventra and the L. bijie was the largest, and the L. purpuraventra and the L. chishuiensis had the smallest difference (Fig. 2); The difference of six species of Leptobrachella mainly focused on the ratio of snout length / snout-vent length, internasal distance / snout-vent length, upper eyelid width / snout-vent length, maximal tibia width / snout-vent length, hindlimb length / snout-vent length, fourth finger length / snout-vent length (Fig. 4). The accuracy rate of morphological feature discrimination was 75.0%﹣100.0%, and the comprehensive discrimination accuracy rate was 90.0%. In addition, we also compared the quality characteristics of six species of Leptobrachella (Table 4), and found that they can also be distinguished by quality characteristics. [Conclusion] This study initially revealed the morphological differences of these six species of Leptobrachella in the Guizhou Plateau. It is believed that the Leptobrachella in the Guizhou Plateau can be well distinguished by measurable and qualitative traits.