[Objectives] The Wuyi Mountain and Meihua Mountain National Nature Reserves in Fujian Province are located in the mid-subtropical monsoon evergreen broad-leaved forest climate zone. This region has high species diversity. However, there are relatively few reports on the species diversity of soil animals (earthworms). We thus conducted an earthworm collection in the Wuyi Mountain and Meihua Mountain National Nature Reserves. [Methods] Morphological taxonomy methods and DNA barcode technology were used to identify the new species. The Bootstrap and P-distance methods were used to calculate the genetic distance between species, and the Bootstrap method and Jukes-Cantor model were used to construct molecular phylogenetic NJ trees in MEGA-X. [Results] Four new earthworm species belonging to the family Megascolecidae and genus Amynthas were found: A. epigenus Sun & Qiu, sp. nov., A. meihuamontis Sun & Qiu, sp. nov., A. muscosus Sun & Jiang, sp. nov. and A. humatalis Sun & Jiang, sp. nov (Fig. 1﹣4). These four new species belong to the A. sieboldi-group in the species group of Sims & Easton, and their spermatheca pores are located in 6/7﹣8/9. We provided morphological descriptions, pictures, morphological comparisons with similar species, and barcode accession numbers submitted to GenBank for all the new species. In addition, we also searched out earthworm species that are closely related to the new species through BLAST on GenBank (Table 1). The genetic distance calculations show that the genetic distance between new species and related species is greater than the interspecies genetic distance (usually less than 1%), and meets the range of intraspecies genetic distance (8.3%﹣13.0%, and above), so they have at least species level Genetic difference (Table 2, 3). The molecular phylogenetic relationship rebuilding result shows that the positions of the four new species in the phylogenetic tree are different from those of other species, which further proves that the four species reported in this paper are new species (Fig. 5). [Conclusion] In summary, the morphological and genetic evidence suggests that A. epigenus Sun & Qiu, sp. nov., A. meihuamontis Sun & Qiu, sp. nov., A. muscosus Sun & Jiang, sp. nov. and A. humatalis Sun & Jiang, sp. nov are new species. The present report has made the number of earthworm species increase from 4 to 8 in Wuyishan and Meihuashan National Nature Reserves in Fujian Province.