Abstract:Two female specimens were trapped by the pitfall trap method in Munike Village, Yangmei Township, Liupanshui City, Guizhou Province, China. This discovery will expand our knowledge about the geographic distribution of Episoriculus. Morphological characterization, and measurements about external and cranial features were used in morphological studies. Genetic distances were calculated and phylogenetic tree was constructed based on the complete sequence of mtDNA Cyt b gene (1 140 bp) in molecular biology research (Table 1). The head and body length of the two specimens are 57.2 mm and 50.4 mm, with their tail lengths (53.2 mm and 46.7 mm) slightly shorter than the head and body lengths. There is little color difference between the back and the ventral surface of the tail, and the ventral surface is slightly brown. Hind feet length is 11.9 mm and 12.4 mm. Condyloincisive length is 17.28 mm and 17.23 mm. Maxillary breadth is 5.02 mm and 4.96 mm. Maxillary breadth to palatoincisive length (7.72 mm and 7.56 mm) is 65.31% (Table 2). There are 1 upper incisor and 4 upper unicuspid teeth. The first and the second unicuspid tooth are similar in size, significantly larger than the third unicuspid tooth. The fourth unicuspid tooth is very tiny, and inserted in the inner side of the gap between the third unicuspid tooth and the premolar tooth. There are 1 premolar and 3 molars in the maxillary, and the third molar is smaller. There are 1 incisor, 1 unicuspid, 1 premolar and 3 molars in the mandible. Based indentification on key to Episoriculus, the specimens collected are determined as E. caudatus umbrinus. The genetic distances between E. c. umbrinus specimen recorded and our specimens were the nearest between 0.030 and 0.062, based on mitochondrial cytochrome b gene. It was shown that our specimen lay in a monophyletic clade together with E. c. umbrinus in the phylogenetic tree, further confirming our identification. Phylogenetic Bayesian tree of the genus Episoriculus shows that E. c. umbrinus has the closest relationship of phylogeny with E. sacratus, followed by E. c. caudatus. The two specimens of E. c. umbrinus are not only the new record of E. caudatus and E. c. umbrinus in Guizhou Province, but also the new record of the genus Episoriculus in Guizhou Province.