[Objectives] During the fieldworks in April-May and July-August 2021, we collected 8 specimens of Amolops (Anura: Ranidae) from Gaoligong Mountain, Yunnan Province, China, which were identified as A. deng and A. chayuensis by comparison of Morphology and Phylogeny. This represents first records of these species in Yunnan Province. [Methods] In this study, the morphological characteristics of the specimens we collected were compared and analyzed with the holotype of A. deng and A. chayuensis, respectively. Based on 16S rRNA gene and COI gene fragments, we used Mrbayes-3.1.2 to construct bayesian tree for phylogenetic analysis. Based on 16S rRNA gene fragments, we used p-distance in MEGA 7 to calculate the genetic distance between individuals of Amolops. All of the new sequences from this study have been uploaded to the NCBI GenBank, the other sequence we used were obtained in NCBI GenBank database (Table 1). [Results] The morphological characteristics are consistent between the specimens collected in this study and holotype. A. deng has a medium body size (SVL 63.1 to 68.5 mm in adult female, n = 3, 49.3 to 52.9 mm in adult male, n = 3); head length is larger than the head width; tympanum is obvious, and taller than the length; three metacarpal tubercles; supratympanic fold is not obvious; dorsolateral fold obvious and connect with the crotch; tibiotarsal articulation beyond snout. A. chayuensis has a small body size (SVL 44.7 mm and 44.8 mm in male, n = 2); males with external subgular vocal sacs; supratympanic fold and dorsolateral fold obviously; arms and legs with brown bands. In addition, the bayesian analyses strongly support the specimens we collected forms a monophyletic clades respectively with each holotype specimens of A. chayuensis and A. deng from Tibet, China (Fig 3). Based on 16S rRNA, the genetic distance of A. deng was 0.8%﹣1.2% and A. chayuensis was 0.2% between the samples we collected and type locality (Table 3). [Conclusion] In summary, the two species of Amolops collected in Gaoligong Mountain in Yunnan are determined to be A. deng and A. chayuensis. Both A. deng and A. chayuensis are first record of Gongshan and Fugong counties of Yunnan province (Fig. 4). In this study, we further updated the elevation range of the distribution of A. chayuensis, from 1 385 m lower to 2 884 m higher than the previous record.