[Objectives] Amphibians and reptiles are important components of biodiversity and important groups of ecosystems. In this study, we investigated the diversity and altitudinal distribution characteristics of amphibians and reptiles in Yingjing Area of the Giant Panda National Park (102°19′﹣102°55′ E, 29°28′﹣29°56′ N). [Methods] From May to September 2021, we set up six observation sites in the Yingjing Area of Giant Panda National Park, and set up 33 effective line transects to investigate the diversity and elevation distribution of amphibians and reptiles, and supplemented the field survey results by interview survey and literature review (Appendix 1). We calculated the Shanoon-Winner’s diversity index, Pielou’s evenness index and ecological dominance index by Spass software (IBM SPSS Statistics 21.0). In addition, Pearson correlation analysis was conducted for these three indices in six study sites. Finally, we analyzed the correlation between the distribution of amphibians and reptiles and the elevation. The statistical test was set two-tailed, and the nominal significance level was set at P = 0.05. [Results] There are 67 species of amphibians and reptiles in the region, including 31 species of amphibians in 9 families of 2 orders and 36 species of reptiles in 9 families of 2 orders. All amphibians are Oriental species except Pelophylax nigromaculatus. There are 26 species of Middle Eastern Ocean reptiles, and only 9 species are widespread species (Appendix 2). By comparing the habitat types of amphibians in the region, we found the local amphibians were different in their adaptability to the environment (Table 1). The Shanoon-Winner’s diversity index, Pielou’s evenness index and ecological dominance index of the six observation sites showed similar change trends with Xinmiao > Paocaowan > Niba mountain > Yunwu mountain > Dashiba > Anjing (Table 2). [Conclusion] The abundance of amphibians decreased gradually with the increase of altitude gradient. Due to the limited number of reptiles, we did not find obvious altitude distribution characteristics (Fig. 3).