Henan locates in the central of China and is characterized with transition area between Palearctic and Oriental regions in zoogeography. However, there is only few investigations of the third species-most abundant order Eulipotyphla, therefore limiting the study of zoogeography. In this study, we aimed to identify three shrews collected from Xinyang City, China, which would enrich the species diversity of Eulipotyphla in Henan Province, China. During October 2013, a shrew (voucher number IBE20131005) was collected from Baichong conservation station (31°36?54? N, 114°51?13? E) of Liankangshan National Nature Reserve, Xinxian, Xinyang, Henan Province, China; and during May 2018 two shrews (voucher number IBE2018100501 and IBE2018100502) were collected from Dahuwan (32°18?57? N, 113°48?5? E), Wujiadian Town, Shihe District, Xinyang, Henan Province, China. Based on the morphological characteristics (Fig.1), the three shrews could be assigned as species from genus Crocidura. In order to identify the taxonomic status, the morphological and skull variables of these shrews were measured and compared with topotype specimen (Table 1), and then phylogenetic relationship (Fig. 2) within genus Crocidura was analyzed using Cyt b sequences and K2P genetic distance was also estimated by using Cyt b sequences. The cranial length (CIL) of the specimen IBE2018100501 is 20.40 mm (Table 1). The cranial width (CB) is 9.62 mm, the orbital width (IOB) is 4.79 mm, the great width measured at anterior labial margins of 2nd upper molars (M2-M2) is 6.16 mm, the upper dentition length (UTRL) is 9.14 mm, and the lower dentition length (LMTR) is 8.13 mm. The genetic distance of K2P between Henan Province and other regions is 0.1%﹣1.2%. Both morphological (Fig. 1) and phylogenetic analyses (Fig. 2) suggested that the three shrews were Crocidura tanakae, a new record of mammals in Henan Province, China. This study also suggested the north-most distribution of C. tanakae was higher than previous record. The C. tanakae collected from the three locations of Mt. Dabieshan showed a very close phylogenetic relationship but further phylogenetic relationship with populations collected from Mt. Huangshan area located close to the south edge of Changjiang River, which suggested the river could be a natural barrier for the gene flows of this species. The specimens (voucher number IBE20131005, IBE2018100501 and IBE2018100502) were stored at the Institute of Biodiversity and Ecology, Zhengzhou University.