[Objectives] Wildlife reintroduction is an important means of protecting endangered populations, but the risk of disease at the release site may affect the health of the introduced species, leading to the failure of reintroduction projects. Disease risk assessment (DRA) is used to identify, prioritize, and design mitigation strategies to address these risks. The purpose of this study was to assess the gastrointestinal parasitic diseases risk of Przewalski’s Horse (Equus przewalskii) reintroduced to the Inner Mongolia Daqingshan National Nature Reserve for the first time, and put forward corresponding prevention and control suggestions. [Methods] On the basis of traditional literature research and combined with field investigation results, this study conducted the expert review, identified, and rated the threat factors, and assessed the risk assessment of parasitic diseases, one of the important factors affecting the health of Przewalski’s Horse population in China. Among them, the field investigation clarified the infection intensity of the eggs, and the molecular identification of the parasite species was carried out by metabarcoding. The taxonomy of each OTU representative sequence was analyzed by BLAST against the Nematode ITS2 v.1.0.0 database using confidence threshold of 0.97. The results of the field survey, such as the distance from the release site, infection rate, infection intensity, and parasite species, were used as the basis for assigning points. [Results] A total of 44 parasites were identified, belonging to 5 classes, 7 orders, 8 families, and 19 genera, causing 10 high-risk parasitic diseases, 13 medium-risk parasitic diseases, and 21 low-risk parasitic diseases (Appendix 3). The domestic horses and donkeys in the reintroduction site carried Parascaris equorum, Cylicocyclus nassatus, Cylicostephanus longibursatus, and Cylicostephanus goldi, which are at higher risk of disease in Przewalski's Horse (Table 3). Xiaojing Village, Kuisu Village. and Dishui Village were all low-risk areas, while Changhan Naobao Village was not banned for grazing and carried Poteriostomum ratzii, which had not been documented and had not been found in Przewalski's Horse parasites worldwide (Table 3). It is therefore identified as a high-risk area. Domestic horses and donkeys around reintroduced sites are at risk of cross-infection with parasites from released Przewalski's Horses, whether in direct or indirect contact. [Conclusion] This study considered that with appropriate disease prevention and control measures, Przewalski's Horse could be reintroduced to the Inner Mongolia Daqingshan National Nature Reserve. It is suggested that the villages in the Inner Mongolia Daqingshan National Nature Reserve should limit the range of activities of domestic horses and donkeys, regularly deworm the livestock, clean stalls, strictly implement the policy of banning grazing, and increase the water source of Przewalski’s Horses far away from villages. Long-term monitoring of parasitic diseases of reintroduced population is also suggested, to effectively prevent the occurrence and spread of parasitic diseases.