Three specimens of Glyphoglossus were collected from Chongqing Wulong Fairy Mountain National Forest Park in May 2021. The specimens were identified as G. yunnanensis (Amphibia, Anura, Microhylidae) by morphological and molecular data, which is a new record of this species in Chongqing municipality. Seventeen morphological characteristics of the specimens were measured (Table 2) and their habitats (Fig. 1) were also described. The construction of maximum likelihood trees for 16S rRNA gene fragments associated with 10 different geographic populations of G. yunnanensis populations showed that the sequences of specimens from Wulong, Chongqing, clustered with those from G. yunnanensis frog locality, and the genetic distance between them was 0.6%, much smaller than that between species of the genus Glyphoglossus (Table 2 and Fig. 2). This new locality in Wulong, Chongqing extends the distribution area of this species northeastward for more than 350 km in China (Fig. 3). The new record of G. yunnanensis in Chongqing will play an important role in understanding the potential distribution area and pedigree geography of this species.