Abstract:In November, 2021, 2 amphibians were collected from Puhe Wetland Park, Liaozhong District, Shenyang (41°30′55″ N, 122°78′30″ E, elevation 30 m). After comparison of morphological characteristics, they were confirmed as species of Rana, Ranidae and Anura.In order to determine the taxonomic relationship of this species and further determine their distribution characteristics, we conducted in-depth research and analysis on them. We used second high-throughput sequencing to sequence the mitochondrial 16S rRNA gene, and constructed a phylogenetic tree to analyze the phylogeny of 19 species of Rana (Table 1). This species of Rana has the closest genetic distance to R. coreana from Korea and Kunyu Mountain in Shandong Province, China, and clustered into a branch in maximum likelihood tree, which suggested an intraspecies relationship (Fig. 2 and Table 2). Based on morphological analysis and phylogenetic comparison, it was determined that the collected specimen was R. coreana (Fig. 1). R. coreana is a new record of amphibians in Liaoning Province. The discovery brings the number of frog species in Liaoning province to 5.