[Objectives] This study aims to understand the biodiversity status of amphibians and reptiles in Changqing Branch Areas of Giant Panda National Park and promote the effective management of the reserve. [Methods] Field investigations were carried out in 2021 May, July and October, and the survey areas were divided into 5 km × 5 km sample areas. Each sample area was set with 1 to 3 survey transect lines, each of which was 500 m to 2 000 m long (Fig. 1). Site coordinates, altitude and habitat types were recorded, and species were identified by morphology and DNA barcode. [Results] 437 amphibian and reptile specimens was obtained, 37 species were identified by morphological characteristics and DNA barcoding, and 14 species were recorded in combination with literature, a total of 51 species of amphibians and reptiles were recorded. Among them, 15 species of amphibians belong to 13 genera, 8 families, 2 orders. There are 36 species of reptiles, belonging to 22 genera, 6 families, 2 orders. 26 species are endemic to China, and 14 species are listed in The Red List of China’s Vertebrates (Appendix 1). The amphibians and reptiles in the reserve are mainly Oriental species, with a small number of widespread species and Palaearctic species. Among the Oriental species, there are 10 amphibians and 21 reptile species, mainly from central China. The composition of amphibian and reptile fauna further indicates that the Qinling Mountains are in the transitional zone between Palaearctic and Oriental boundaries (Table 1). The diversity index of amphibians and reptiles in Changqing Branch Areas is high and the species diversity is complex. Amphibians and reptiles all had higher rank meta-diversity at family level and lower rank meta-diversity at genus level. In addition, the indexes of reptiles were higher than those of amphibians, indicating that the diversity of reptile family and genus in Changqing Branch Areas was higher than that of amphibians (Table 2). The Oriental species are dominant in different vertical altitudes, and they are concentrated at an altitude of 500﹣2 000 m. In the Palaearctic, the number of widely distributed species also concentrated below 2 000 m (Table 3). Therefore, the core distribution area of amphibians and reptiles in Changqing Branch Area is 500﹣2 000 m above sea level. [Conclusion] 15 species of amphibians and 36 species of reptiles were recorded, accounting for 53.6% and 63.2% of the total number of amphibians and reptiles in Shaanxi Province. Compared with Changqing Reserve, Huangguanshan Reserve, Sangyuan Reserve comprehensive scientific investigation, 5 species of amphibians and 5 species of reptiles were newly added. This survey is helpful to fully understand the general situation of amphibian and reptile resources in Changqing Branch Areas of Giant Panda National Park, and provide basic information for scientific conservation and management.