During the amphibian survey in Xueshan Town, Weining County, Guizhou Province, China on 6 August, 2020, six specimens were collected (27°04′09″ N, 104°04′44″ E, altitude 2 218 m). Compared the specimens with other species of the genus Odorrana (Table 1) by morphological and molecular evidences, the specimens were identified as O. jingdongensis, which is the new record of Guizhou Province, China. Morphologically, we mainly compared measurement data (Table 2) and diagnosis characters (Fig. 1), the result showed that the specimens from Guizhou were similar to O. jingdongensis. Molecularly, we constructed Bayesian inference (BI) and maximum likelihood (ML) inference phylogenetic trees based on 16S rRNA gene and calculated the genetic distances between species of the genus Odorrana using Kimura two-parameter model in MEGA v6.0. The ML and BI trees of the mitochondrial DNA dataset presented almost consistent topology, both showed that the four specimens from Guizhou were clustered with the O. jingdongensis with a high support (0.97/92, BI/ML) (Fig. 2). The genetic distance between the specimens from Guizhou and O. jingdongensis from type locality was 0%, which is much lower than that between Odorrana species range from 1.3% to 9.5% (Table 3).