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张方,陈旭,何志新.2012.商城肥鲵肝及胰的解剖学与组织学观察.动物学杂志,47(3):95-101.
商城肥鲵肝及胰的解剖学与组织学观察
Anatomical and Histological Observation on the Liver and Pancreas of Pachyhynobius shangchengensis
投稿时间:2011-11-27  修订日期:2012-02-27
DOI:
中文关键词:  商城肥鲵    胆囊:胰  解剖学  组织学
英文关键词:Pachyhynobius shangchengensis  Liver  Gallbladder  Pancreas  Anatomy  Histology
基金项目:安徽师范大学博士启动基金项目(No.160-750817),重要生物资源的保护及利用安徽省重点实验室基金资助
作者单位E-mail
张方 安徽师范大学生命科学学院 芜湖 241000 biologyzhf@hotmail.com 
陈旭 安徽师范大学生命科学学院 芜湖 241000  
何志新 安徽师范大学生命科学学院 芜湖 241000  
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中文摘要:
      利用常规H.E和改良甲苯胺蓝染色方法(modified toluidine staining,MTB),对商城肥鲵(Pachyhynobius shangchengensis) 肝、胆囊和胰的解剖学、组织学以及上述器官的肥大细胞进行了观察。结果表明,商城肥鲵的肝小叶不明显,肝索互相连接成网状,肝细胞核大,单核,少数2~3核,肝侧缘细胞胞体大,有的达70 μm,但核质比小。肝中存在大量的色素细胞。胆囊黏膜上皮为单层扁平或立方上皮,未见形成皱襞。胰中结缔组织不发达,细胞界限不清,胞核大。除肝的门管区、肝门静脉区和胰的胰管周围分布有较多的肥大细胞外,肝和胰中其他区域分布很少,胆囊壁内有较多的肥大细胞。
英文摘要:
      The anatomy and histology of liver,gallbladder and pancreas in Pachyhynobius shangchengensis were studied by routine H.E staining and mast cells (MCs) in the above organs were observed by modified toluidine staining.The results indicated that hepatic lobules were not evident,and the liver bundles interconnected into reticular structure.The nucleoli of hepatic cells were large.Most hepatic cells were mononuclear,but small proportion of hepatic cells were binuclear and tri-nuclear.The cell body in the hepatic side was bulky,and some up to 70 μm,but the ratio of the nucleus-cytoplasm was small.There were lots of chromatophore cells in the liver.The epithelium cells of gall bladder were simple squamous or cuboidal epithelium,but plica could not be observed.The connective tissue in the pancreas was underdeveloped.The border of the pancreatic cells was not circumscribed and their nuclei were larger.There were more MCs distributed in portal area,in hepatic portal vein and around pancreatic duct,but less MCs in other areas of liver and pancreas.However,there were more MCs distributed in the gallbladder wall.
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